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Image Search Results
Journal: bioRxiv
Article Title: Rotavirus as an Expression Platform of the SARS-CoV-2 Spike Protein
doi: 10.1101/2021.02.18.431835
Figure Lengend Snippet: (A) S protein trimers are cleaved at the S1/S2 junction by furin proconvertase and at the S2’ site by the TMPRSS2 serine protease. The S1 fragment contains a signal sequence (SS), N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The S2 fragment contains a trimeric core region, transmembrane anchor (TM), and fusion domain. (B) Portions of the S protein expressed by recombinant rotaviruses are indicated. (C) Ribbon representations of the closed conformation of the trimeric S protein (PDB 6VXX) showing locations of the RBD (magenta), extended RBD (ExRBD, cyan), NTD (blue), core (CR, gold) domains and the S1 cleavage product (green).
Article Snippet: The RBD antibody recognized the fExRBD product of the rSA11/NSP3-fExRBD virus, but not the fRBD product of rSA11/NSP3-fRBD , presumably because the latter product lacked the peptide sequence used in generating the
Techniques: Sequencing, Binding Assay, Recombinant
Journal: bioRxiv
Article Title: Rotavirus as an Expression Platform of the SARS-CoV-2 Spike Protein
doi: 10.1101/2021.02.18.431835
Figure Lengend Snippet: Illustration indicates nucleotide positions of the coding sequences for NSP3, porcine teschovirus 2A element, 3xFLAG (FL), and the complete S1 or portions of the S1 (NTD, ExRBD, and RBD) and S2 (CR) proteins. The red arrow notes the position of the 2A translational stop-restart site, and the asterisk notes the end of the ORF. Sizes (aa) of encoded NSP3 and S products are in parenthesis. T7 (T7 RNA polymerase promoter sequence), Rz (Hepatitis D virus ribozyme), UTR (untranslated region).
Article Snippet: The RBD antibody recognized the fExRBD product of the rSA11/NSP3-fExRBD virus, but not the fRBD product of rSA11/NSP3-fRBD , presumably because the latter product lacked the peptide sequence used in generating the
Techniques: Sequencing
Journal: bioRxiv
Article Title: Rotavirus as an Expression Platform of the SARS-CoV-2 Spike Protein
doi: 10.1101/2021.02.18.431835
Figure Lengend Snippet: (A, B) Whole cell lysates (WCL) were prepared from cells infected with rSA11 viruses and examined by immunoblot assay using (A) FLAG antibody to detect S products (NTD, ExRBD, RBD, CR, S1, and 2A read-through products) and antibodies specific for rotavirus NSP3 and VP6 and cellular PCNA. Red asterisks identify 2A read-through products and blue asterisks identify 2A cleavage products. (B) Lysates prepared from MA104 cells infected with rSA11wt, rSA11/NSP3-fRBD and rSA11/NSP3-fExRBD were examined by immunoblot assay using antibodies specific for RBD (ProSci 9087), rotavirus VP6, and PCNA. (C) Lysates prepared from MA104 cells infected with rSA11/wt, rSA11/NSP3-fRBD and rSA11/NSP3-fExRBD viruses were examined by immunoprecipitation assay using a SARS-CoV-2 S1 specific monoclonal antibody (GeneTex CR3022). Lysates were also analyzed with a NSP2-specific polyclonal antibody. Antigen-antibody complexes were recovered using IgA/G beads, resolved by gel electrophoresis, blotted onto nitrocellulose membranes, and probed with FLAG (fRBD and fExRBD) and NSP2 antibody. Molecular weight markers are indicated (kDa). Red arrows indicate fRBD and fExRBD. fRBD comigrates near the Ig light chain (Ig/L). Ig heavy chain, Ig/H).
Article Snippet: The RBD antibody recognized the fExRBD product of the rSA11/NSP3-fExRBD virus, but not the fRBD product of rSA11/NSP3-fRBD , presumably because the latter product lacked the peptide sequence used in generating the
Techniques: Infection, Western Blot, Immunoprecipitation, Nucleic Acid Electrophoresis, Molecular Weight
Journal: bioRxiv
Article Title: Rotavirus as an Expression Platform of the SARS-CoV-2 Spike Protein
doi: 10.1101/2021.02.18.431835
Figure Lengend Snippet: Primers used to produce pT7/NSP3–2A-CoV2 plasmids.
Article Snippet: The RBD antibody recognized the fExRBD product of the rSA11/NSP3-fExRBD virus, but not the fRBD product of rSA11/NSP3-fRBD , presumably because the latter product lacked the peptide sequence used in generating the
Techniques: Sequencing, Plasmid Preparation